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Overview
1.1

### Identified secondary metabolite regions using strictness 'relaxed'

BGC0001042 (Streptomyces flaveolus)

Region | Type | From | To | Similarity Confidence | Most similar known cluster
--- | --- | --- | --- | --- | ---
Region 1 | NRPS,T1PKS,hglE-KS,PKS-like | 1 | 118,372 | High | sanglifehrin A | NRPS:Type I+PKS

BGC0001042 - Region 1 - NRPS,PKS-like,T1PKS,hglE-KS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1 - 118,372 nt. (total: 118,372 nt) Show pHMM detection rules used
Region on contig edge.
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
hglE-KS: (hglE or hglD)
PKS-like: (t2pks2 or ksIII)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
binding site
reset view
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Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
Tailoring
MIBiG comparison
ClusterBlast
KnownClusterBlast
SubClusterBlast
TFBS Finder
NRPS/PKS modules
Pfam domains
TIGRFAM domains
NRPS/PKS domains
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Tailoring functions
Tailoring enzymes detected in this region
Oxidoreductases: dehydrogenase oxygenase
ACY06266.1: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

ACY06271.1: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

ACY06273.1: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs
  SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ACY06308.1: monooxygenase FAD-binding

smcogs
  SMCOG1050: monooxygenase FAD-binding

sfaB: lysine/ornithine N-monooxygenase

smcogs
  SMCOG1080: lysine/ornithine N-monooxygenase

sfaJ: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs
  SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

sfaM: short-chain dehydrogenase/reductase SDR

smcogs
  SMCOG1001: short-chain dehydrogenase/reductase SDR

sfaR: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs
  SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acylase methyltransferase
ACY06270.1: methyltransferase

smcogs
  SMCOG1089: methyltransferase

sfaI: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

sfaL: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase hydrolase
sfaP: asparagine synthase (glutamine-hydrolyzing)

smcogs
  SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

sfaQ: thioesterase

smcogs
  SMCOG1004: thioesterase

Ligases: ligase synthase
ACY06269.1: AMP-dependent synthetase and ligase

smcogs
  SMCOG1002: AMP-dependent synthetase and ligase

sfaD: AMP-dependent synthetase and ligase

smcogs
  SMCOG1002: AMP-dependent synthetase and ligase

sfaF: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

sfaG: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

sfaH: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

sfaK: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

sfaN: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs
  SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | NRPS | T1PKS | hglE-KS | PKS-like | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | --- | --- | --- | ---
BGC0001042.5 |  |  |  |  | 2.01 | NRPS, PKS | sanglifehrin A | Streptomyces flaveolus
BGC0001275.3 |  |  |  |  | 1.25 | PKS | 6-methylsalicyclic acid | Glarea lozoyensis
BGC0001244.3 |  |  |  |  | 1.23 | PKS | (-)-Mellein | Parastagonospora nodorum
BGC0002192.3 |  |  |  |  | 1.15 | PKS | FR901512 | fungal sp. No.14919
BGC0001284.4 |  |  |  |  | 1.13 | PKS | alternariol | Parastagonospora nodorum SN15
BGC0001254.3 |  |  |  |  | 1.12 | PKS | ACT-Toxin II | Alternaria alternata
BGC0001164.3 |  |  |  |  | 1.11 | PKS | 1-heptadecene | Cyanothece sp. PCC 7822
BGC0001160.5 |  |  |  |  | 1.10 | PKS | 1-nonadecene, (14Z)-1,14-nonadecadiene | Synechococcus sp. PCC 7002
BGC0001268.4 |  |  |  |  | 1.06 | NRPS, PKS | fusarin C | Fusarium fujikuroi
BGC0000041.3 |  |  |  |  | 1.00 | PKS | oronofacic acid | Pseudomonas syringae

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0001042.5 |  | 1.00 | NRPS, PKS | sanglifehrin A | Streptomyces flaveolus
BGC0002135.2 |  | 0.76 | NRPS | bovienimide A | Xenorhabdus bovienii SS-2004
BGC0001833.4 |  | 0.75 | NRPS | icosalide A, icosalide B | Burkholderia gladioli
BGC0001273.3 |  | 0.74 | PKS | asperlactone | Aspergillus ochraceus
BGC0001276.3 |  | 0.74 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001135.5 |  | 0.73 | NRPS | bicornutin A1, bicornutin A2 | Xenorhabdus budapestensis
BGC0001275.3 |  | 0.73 | PKS | 6-methylsalicyclic acid | Glarea lozoyensis
BGC0002286.3 |  | 0.73 | NRPS | ririwpeptide A, ririwpeptide B, ririwpeptide C | Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4 |  | 0.73 | NRPS | gamexpeptide C | Photorhabdus laumondii subsp. laumondii TTO1
BGC0001244.3 |  | 0.73 | PKS | (-)-Mellein | Parastagonospora nodorum

Similar known clusters
Shows regions from the antiSMASH database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on a reference name to open that entry in the antiSMASH database (if applicable).
Location:
Identifier
Identity
Coverage
Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.
Location:
Identifier
Identity
Coverage
Similar subclusters
Shows sub-cluster units that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.

No significant matches found.

Transcription Factor Binding Sites
Detailed information for Transcription Factor Binding Site hits, including surrounding genes where applicable.
The strand of the match is shown by an arrow underneath the match.
Detailed documentation is available here.
Binding sites found:
IolR
Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.54 out of 23.79
Confidence: medium
AbrC3
Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16
Confidence: strong
VqsM
Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47
Confidence: medium
GnfM
Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.64 out of 27.55
Confidence: medium
Additional weak hits (15):
ToxT
Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.04 out of 18.57
Confidence: weak
ClgR
Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.54 out of 30.22
Confidence: weak
MexT
Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.53 out of 21.84
Confidence: weak
ANR
Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.82 out of 26.33
Confidence: weak
ColR
Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.84 out of 30.25
Confidence: weak
FuR variant 1
Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.31 out of 29.38
Confidence: weak
PerR
Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.33 out of 28.14
Confidence: weak
CcpA
Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.82 out of 20.97
Confidence: weak
MexT
Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84
Confidence: weak
MexT
Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84
Confidence: weak
ANR
Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33
Confidence: weak
HgtR
Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.25 out of 36.54
Confidence: weak
HgtR
Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.10 out of 36.54
Confidence: weak
HypR
Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40
Confidence: weak
NrdR
Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.46 out of 27.18
Confidence: weak
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
Detailed Pfam domain annotation
Shows Pfam domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description. Domains with a bold border have Gene Ontology information.
Selected features only
Expand to show all names
Detailed TIGRFAM domain annotation
Shows TIGRFAM domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description.
Selected features only
Expand to show all names
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
NRPS/PKS products
NRPS/PKS substrates
TFBS Finder
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 1, location 0 - 118372:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (Val - X - X) + (Me-redmal) + (Me-mal - Me-ccmal - ohmal) + (Me-ohmal - redmal) + (ccmal - Me-ccmal - ohmal - ccmal - ccmal) + (Me-ohmal - ccemal) + (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 116040:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (Val - X - X) + (Me-redmal) + (Me-mal - Me-ccmal - ohmal) + (Me-ohmal - redmal) + (ccmal - Me-ccmal - ohmal - ccmal - ccmal) + (Me-ohmal - ccemal) + (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 77395 - 118372:
Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:
  (ccmal - Me-ccmal - ohmal - ccmal - ccmal) + (Me-ohmal - ccemal) + (mal)
Non-colinear ordering:
  sfaH + sfaI + sfaK

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 84000 - 118372:
Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:
  (ccmal - Me-ccmal - ohmal - ccmal - ccmal) + (Me-ohmal - ccemal) + (mal)
Non-colinear ordering:
  sfaH + sfaI + sfaK

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).
ACY06269.1: fatty_acid
CAL_domain (31..464): fatty_acid
minowa: fatty_acid

Prediction, score:
  fatty_acid: 23.6
  AHBA: 16.3
  shikimic_acid: 9.1
  NH2: 5.9
  Acetyl-CoA: 0.0

sfaD: Val - X - X
Search NORINE for peptide: strict or relaxed
AMP-binding (498..892): Val
nrpys: Val

SVM prediction details:
  Predicted physicochemical class:
    hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
  Large clusters prediction:
    Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
  Small clusters prediction:
    Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
  Single AA prediction:
    val (Val)

Stachelhaus prediction details:
  Stachelhaus sequence:
    DAYWVGGTFK
  Nearest Stachelhaus code(s):
    Val DAYWVGGTFK (100% 8Å match)
  Stachelhaus code match:
    100% (strong)

AMP-binding (1537..1927): X
nrpys: mTyr

SVM prediction details:
  Predicted physicochemical class:
    hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
  Large clusters prediction:
    Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
  Small clusters prediction:
    N/A
  Single AA prediction:
    N/A

Stachelhaus prediction details:
  Stachelhaus sequence:
    DIYCLGAAFK
  Nearest Stachelhaus code(s):
    mTyr DIYCLGAAFK (100% 8Å match)
  Stachelhaus code match:
    100% (strong)

AMP-binding (2579..2980): X
nrpys: Piz

SVM prediction details:
  Predicted physicochemical class:
    hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
  Large clusters prediction:
    Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
  Small clusters prediction:
    pro (Pro)
  Single AA prediction:
    pro (Pro)

Stachelhaus prediction details:
  Stachelhaus sequence:
    DVQFSAHVVK
  Nearest Stachelhaus code(s):
    Piz DVQFSAHVVK (100% 8Å match)
  Stachelhaus code match:
    100% (strong)

sfaE: mmal
PKS_AT (652..949): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 218.4
  Ethylmalonyl-CoA: 208.0
  Methoxymalonyl-CoA: 182.4
  trans-1,2-CPDA: 137.9
  2-Methylbutyryl-CoA: 137.4
  Isobutyryl-CoA: 105.5
  CHC-CoA: 105.2
  Propionyl-CoA: 103.3
  Malonyl-CoA: 95.1
  Benzoyl-CoA: 90.9
  Acetyl-CoA: 55.6
  3-Methylbutyryl-CoA: 49.5
  fatty_acid: 36.8
  inactive: 24.0

sfaF: mmal - mmal - mal
PKS_AT (563..857): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 222.9
  Ethylmalonyl-CoA: 209.2
  Methoxymalonyl-CoA: 183.0
  2-Methylbutyryl-CoA: 142.9
  trans-1,2-CPDA: 134.5
  CHC-CoA: 115.5
  Propionyl-CoA: 113.7
  Malonyl-CoA: 109.1
  Isobutyryl-CoA: 104.8
  Benzoyl-CoA: 82.8
  Acetyl-CoA: 55.1
  3-Methylbutyryl-CoA: 46.2
  inactive: 31.1
  fatty_acid: 27.1

PKS_AT (1573..1868): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 218.9
  Ethylmalonyl-CoA: 206.6
  Methoxymalonyl-CoA: 180.7
  2-Methylbutyryl-CoA: 152.3
  trans-1,2-CPDA: 127.9
  CHC-CoA: 119.2
  Propionyl-CoA: 115.8
  Malonyl-CoA: 113.5
  Isobutyryl-CoA: 96.2
  Benzoyl-CoA: 68.9
  3-Methylbutyryl-CoA: 42.5
  Acetyl-CoA: 41.2
  fatty_acid: 34.1
  inactive: 32.4

PKS_AT (3230..3500): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 75.0%
  Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 184.2
  inactive: 83.7
  Methoxymalonyl-CoA: 65.7
  Methylmalonyl-CoA: 59.1
  Isobutyryl-CoA: 36.5
  Propionyl-CoA: 33.3
  fatty_acid: 22.9
  Benzoyl-CoA: 20.1
  trans-1,2-CPDA: 19.7
  Acetyl-CoA: 18.1
  2-Methylbutyryl-CoA: 16.6
  CHC-CoA: 7.5
  Ethylmalonyl-CoA: -3.1
  3-Methylbutyryl-CoA: -3.9

sfaG: mmal - mal
PKS_AT (561..850): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 91.7%
  Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 197.2
  Ethylmalonyl-CoA: 182.0
  Methoxymalonyl-CoA: 172.2
  2-Methylbutyryl-CoA: 120.2
  Malonyl-CoA: 106.6
  trans-1,2-CPDA: 102.2
  Propionyl-CoA: 100.3
  CHC-CoA: 93.9
  Benzoyl-CoA: 67.6
  Isobutyryl-CoA: 53.6
  Acetyl-CoA: 37.0
  3-Methylbutyryl-CoA: 35.1
  fatty_acid: 25.5
  inactive: 18.9

PKS_AT (2049..2344): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 79.2%
  Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 188.6
  inactive: 89.3
  Methoxymalonyl-CoA: 72.9
  Methylmalonyl-CoA: 60.5
  Propionyl-CoA: 39.8
  Ethylmalonyl-CoA: 22.9
  Acetyl-CoA: 22.8
  Isobutyryl-CoA: 22.1
  2-Methylbutyryl-CoA: 19.5
  Benzoyl-CoA: 19.1
  CHC-CoA: 17.9
  fatty_acid: 14.8
  trans-1,2-CPDA: 6.7
  3-Methylbutyryl-CoA: 0.0

sfaH: mal - mmal - mal - mal - mal
PKS_AT (558..844): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 75.0%
  Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 180.8
  inactive: 83.8
  Methoxymalonyl-CoA: 80.3
  Methylmalonyl-CoA: 66.6
  Isobutyryl-CoA: 54.7
  Ethylmalonyl-CoA: 41.6
  Propionyl-CoA: 38.3
  2-Methylbutyryl-CoA: 28.1
  fatty_acid: 21.4
  Benzoyl-CoA: 18.7
  3-Methylbutyryl-CoA: 15.1
  Acetyl-CoA: 12.6
  trans-1,2-CPDA: 11.6
  CHC-CoA: 0.0

PKS_AT (2254..2548): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 224.7
  Ethylmalonyl-CoA: 209.2
  Methoxymalonyl-CoA: 185.9
  2-Methylbutyryl-CoA: 150.8
  trans-1,2-CPDA: 129.3
  CHC-CoA: 116.9
  Propionyl-CoA: 109.4
  Malonyl-CoA: 106.2
  Isobutyryl-CoA: 104.7
  Benzoyl-CoA: 87.3
  Acetyl-CoA: 55.8
  3-Methylbutyryl-CoA: 53.3
  inactive: 31.9
  fatty_acid: 31.7

PKS_AT (3928..4198): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 75.0%
  Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 174.2
  inactive: 80.3
  Methoxymalonyl-CoA: 68.3
  Methylmalonyl-CoA: 62.8
  Isobutyryl-CoA: 38.0
  Propionyl-CoA: 37.4
  fatty_acid: 25.5
  Benzoyl-CoA: 22.8
  trans-1,2-CPDA: 21.6
  Acetyl-CoA: 19.0
  2-Methylbutyryl-CoA: 17.3
  CHC-CoA: 9.9
  Ethylmalonyl-CoA: -3.1
  3-Methylbutyryl-CoA: -3.6

PKS_AT (5378..5648): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 75.0%
  Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 176.9
  inactive: 84.8
  Methoxymalonyl-CoA: 69.4
  Methylmalonyl-CoA: 64.1
  Isobutyryl-CoA: 38.7
  Propionyl-CoA: 35.4
  Benzoyl-CoA: 25.9
  fatty_acid: 25.6
  trans-1,2-CPDA: 23.4
  2-Methylbutyryl-CoA: 22.7
  Acetyl-CoA: 18.5
  CHC-CoA: 8.8
  3-Methylbutyryl-CoA: 0.0
  Ethylmalonyl-CoA: -3.1

PKS_AT (7048..7335): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 79.2%
  Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 193.8
  inactive: 100.2
  Methoxymalonyl-CoA: 60.9
  Methylmalonyl-CoA: 56.4
  Propionyl-CoA: 37.0
  Isobutyryl-CoA: 32.6
  Ethylmalonyl-CoA: 29.6
  2-Methylbutyryl-CoA: 24.2
  fatty_acid: 23.2
  Benzoyl-CoA: 21.7
  Acetyl-CoA: 14.5
  CHC-CoA: 13.7
  trans-1,2-CPDA: 5.7
  3-Methylbutyryl-CoA: 0.0

sfaI: mmal - emal
PKS_AT (631..924): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 213.4
  Ethylmalonyl-CoA: 207.5
  Methoxymalonyl-CoA: 173.8
  2-Methylbutyryl-CoA: 152.1
  trans-1,2-CPDA: 124.5
  Propionyl-CoA: 120.8
  Malonyl-CoA: 111.2
  CHC-CoA: 107.1
  Isobutyryl-CoA: 83.1
  Benzoyl-CoA: 53.1
  3-Methylbutyryl-CoA: 48.2
  Acetyl-CoA: 39.8
  inactive: 36.9
  fatty_acid: 28.1

PKS_AT (2188..2486): emal
ATSignature: Ethylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:
  Ethylmalonyl-CoA: 87.5%
  Methoxymalonyl-CoA: 87.5%
  Methylmalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 203.9
  Methylmalonyl-CoA: 184.1
  Methoxymalonyl-CoA: 158.5
  trans-1,2-CPDA: 119.3
  2-Methylbutyryl-CoA: 114.2
  Propionyl-CoA: 90.8
  Malonyl-CoA: 83.8
  Isobutyryl-CoA: 83.1
  CHC-CoA: 78.2
  Benzoyl-CoA: 72.0
  Acetyl-CoA: 38.2
  fatty_acid: 33.0
  3-Methylbutyryl-CoA: 20.0
  inactive: 15.7

sfaK: mal
PKS_AT (542..828): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 75.0%
  inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 54.0
  Methylmalonyl-CoA: 21.5
  Methoxymalonyl-CoA: 20.9
  inactive: 16.3
  Ethylmalonyl-CoA: 3.8
  CHC-CoA: 3.2
  fatty_acid: 2.8
  Isobutyryl-CoA: 2.2
  Propionyl-CoA: 0.5
  trans-1,2-CPDA: 0.0
  Benzoyl-CoA: 0.0
  Acetyl-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0
  2-Methylbutyryl-CoA: 0.0

sfaL: mal
PKS_AT (120..329): mal
ATSignature: (unknown)
No matches above 50%
minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 0.5
  trans-1,2-CPDA: 0.0
  inactive: 0.0
  fatty_acid: 0.0
  Propionyl-CoA: 0.0
  Methylmalonyl-CoA: 0.0
  Methoxymalonyl-CoA: 0.0
  Isobutyryl-CoA: 0.0
  Ethylmalonyl-CoA: 0.0
  CHC-CoA: 0.0
  Benzoyl-CoA: 0.0
  Acetyl-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0
  2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor
TFBS definitions
Shows descriptions for Transcription Factor Binding Site models

Regulator | Description
--- | ---
AbrC3 | Antibiotic production activator
GnfM | Regulator of nitrogen-fixation genes
IolR | Regulator of the inositol catabolism
VqsM | Regulator of quorum-sensing signalling systems